COURSE DESCRIPTION
While you
are reading this text, billions of neurons in your brain are electrically
talking to each other. All your thoughts and movements are generated by the
coordinated movement of electrically charged particles into and out of these
neurons. Therefore, electricity in the brain forms the basis of who we are.
In the
course “Experimental Neurophysiology – theory and practice”, you will learn
about experimental techniques that you can use to measure the tiny currents and
voltage changes in the brain. These range from recording signals from within
single neurons by using tiny glass electrodes, to recording the summed activity
of thousands of neurons simultaneously, using extracellular recording
electrodes. By starting at the single neuron level, and slowly moving up to
networks of neurons during the week, you will get a full overview of what we
can measure, and the challenges that come with each level.
Additionally, the afternoons will consist of hands-on experiments at each of these different levels. Finally, the course will end the week with an interactive masterclass with a world-renowned expert in this field from the USA. The course will be focused on recording electrical activity in the rodent brain.
Together, this course should help you in interpreting electrophysiological experiments related to your own research project, be it in literature, seminars or discussions with peers. Additionally we hope that it will inspire you to perhaps implement such experiments in your own research, or to set up collaborations with other electrophysiologists.
LEARNING GOALS
After following this course students will be able to:
- Describe state-of-the-art electrophysiological principles and methods
- Indicate which electrophysiological techniques are suitable for answering research questions (single channel, single cell, network, in vivo)
- Critically evaluate research papers containing electrophysiological recordings, formulate questions about methodology and interpretation, and discuss these.
- Generate ideas about how electrophysiological techniques can enrich their research, and which techniques are most suitable.
CONTENTS
The following topics will be covered during the course:
- Principles of neuronal excitability
- Cable theory and Action Potentials
- Neuron modelling
- In vitro whole-cell patch-clamp recordings
- Synaptic transmission and plasticity
- In vivo recordings (patch-clamp, juxtacellular recording, single-unit recording)
- Optogenetics
REQUIRED BACKGROUND LEVEL
The course is open to all ONWAR PhD students. A solid background in general neuroscience is expected. However, we specifically encourage students with limited experience with / knowledge of experimental neurophysiology to apply. Background reading will be distributed several weeks before the course starts, with the aim to reduce the often large differences in background knowledge between students.
FORMAT
The course consists mainly of Lectures and practical work. In addition, a short writing assignment and a masterclass are integrated into the course program.
DURATION
40 hrs / 1.4 ECTs
ORGANISERS
Dr. Rogier Min (VUmc)
Prof. dr. Maarten Kole (NIN, UU)
Dr. Niels Cornelisse (VU)
Prof. dr. Gerard Borst (EUR)
DATES
October 21-25, 2024
LOCATION
NIN, VU, Erasmus MC
PROGRAM
Click here for the program of the previous edition
REGISTRATION
To register, please send an e-mail to registration@onwar.nl, including a short motivation stating:
• Why you would like to participate in the course. • What you expect from the course. • What your background is