COURSE DESCRIPTION
This intensive course of one week is organised by the Erasmus Optical Imaging Centre. It gives an overview of the rapidly developing advanced fluorescence imaging methods and how they are used in biomedical research. An important part is the practical work each afternoon in which you get the opportunity for real hands-on experience. The focus of the practicals is on functional imaging (time lapse, FRAP, FRET) and super resolution (SIM, STED and single molecule tracking).
LEARNING GOALS
To get insight in and hands-on experience with advanced fluorescence imaging methods and data analysis
CONTENTS
The following topics will be covered during the course:
Lectures
- Confocal Microscopy & Live Cell Imaging
- FRET, FRAP, TIRF, FLIM
- Super Resolution (SIM, STED, PALM/STORM, Rescan)
- Deconvolution
- Fluorescent Proteins
- Biological Applications
- Image Analysis
Hands-on Practical Work
- Introduction to Confocal Microscopy
- Time Lapse Imaging
- FRAP
- Super Resolution Imaging (SIM, STED, PALM/STORM/Localisation Mic., Rescan, lightsheet)
- Data Analysis
- Data Presentation
FORMAT
This five day course consists of lecture sessions in the morning and practical sessions in the afternoon. On Monday and Thursday there are also evening sessions with dinner. In the final session on Friday afternoon participants present and discuss the results of one of the topics during the practical work.
REQUIRED BACKGROUND LEVEL
No prior knowledge is required
DURATION
50 hrs / 1.8 ECT
ORGANISERS
Erasmus Optical Imaging Centre (OIC)
Dr. Gert-Jan Kremers
DATES
11-15 November 2024
REGISTRATION
To register, please send your motivation to registration@onwar.nl*,
*please read through the cancellation policy carefully before booking.